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biotinylated goat anti mouse igm  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated goat anti mouse igm
    Biotinylated Goat Anti Mouse Igm, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/BA-2020-%2E5/Biotinylated+Goat+Anti-Mouse+IgM+Antibody%2C+mu+chain+specific/pm40473739-214-0-9
    Average 93 stars, based on 396 article reviews
    biotinylated goat anti mouse igm - by Bioz Stars, 2026-10
    93/100 stars

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    Blocking Assay:

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies
    Article Snippet: .. Biotinylated goat anti-mouse IgM, Mu chain specific secondary antibody (Vector Laboratories, Newark, CA, USA) diluted 1:500 in blocking solution (1F10), or biotinylated goat anti-mouse IgG diluted 1:1000 (AT100) or 1:3000 (7F2 and AT180) was added to the sections for 1 h at RT. .. ImmPRESS HRP horse anti-mouse IgG polymer (Vector Laboratories, Newark, CA, USA) diluted 1:10 was also added to the secondary antibody solution, except for mouse tissue sections immunostained with 7F2.

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies.
    Article Snippet: .. Biotinylated goat anti-mouse IgM, Mu chain specific secondary antibody (Vector Laboratories, Newark, CA, USA) diluted 1:500 in blocking solution (1F10), or biotinylated goat anti-mouse IgG diluted 1:1000 (AT100) or 1:3000 (7F2 and AT180) was added to the sections for 1 h at RT. .. ImmPRESS HRP horse anti-mouse IgG polymer (Vector Laboratories, Newark, CA, USA) diluted 1:10 was also added to the secondary antibody solution, except for mouse tissue sections immunostained with 7F2.

    Amplification:

    Article Title: FGFR3-induced Y158 PARP1 phosphorylation promotes PARP inhibitor resistance via BRG1/MRE11-mediated DNA repair in breast cancer models
    Article Snippet: Slides were blocked with 3% hydrogen peroxide, BLOXALL blocking solution (Vector Laboratories, catalog SP-6000), and normal goat serum before being incubated with primary antibody against p-Y158 PARP1 (clone 2G9.2, 1:50) or p-FGFR (Biorbyt, catalog orb156865; 1:25). .. Biotinylated goat anti-mouse IgM antibody (Vector Laboratories, catalog BA-2020) and ABC-HRP kit (Vector Laboratories, catalog PK-6100) were used for signal amplification. .. Zeiss Zen software was used with a Zeiss LSM 710 confocal microscope for immunofluorescence staining imaging.

    Article Title: FGFR3-induced Y158 PARP1 phosphorylation promotes PARP-inhibitor resistance via BRG1/MRE11-mediated DNA repair in breast cancer models
    Article Snippet: 556 Slides were blocked with 3% hydrogen peroxide, BLOXALL® blocking solution (Vector Laboratories, 557 Cat#SP-6000) and normal goat serum before incubated with primary antibody against p-Y158 PARP1 558 (clone 2G9.2, 1:50) or p-FGFR (Biorbyt, Cat# orb156865, 1:25). .. Biotinylated goat anti-mouse IgM 559 antibody (Vector Laboratories, CatBA-2020) and ABC-HRP kit (Vector Laboratories, Cat#PK-6100) 560 were used for signal amplification. .. 561 Statistics 562 For Western blot signal quantification, signal intensities were analyzed using Image Studio Lite 563 (version 5.2; LI-COR Biosciences).

    Immunohistochemistry:

    Article Title: Cell transplantation-mediated dystrophin supplementation efficacy in Duchenne muscular dystrophy mouse motor function improvement demonstrated by enhanced skeletal muscle fatigue tolerance
    Article Snippet: , Mouse IgG1 (γ1), Alexa Fluor ® 488 conjugated , Goat IgG (polyclonal) , 1: 500 (IHC) , Room temperature, 1 h (IHC) , A21121, Thermo Fisher Scientific. .. , Mouse IgM (mu chain specific), biotinylated , Goat IgG (polyclonal) , 1: 100 (IHC) , Room temperature, 45 min (IHC) , BA-2020, Vector Laboratories. .. Mouse IgG, peroxidase conjugated , Horse , 1: 20,000 (WB) , Room temperature, 1 h (WB) , PI-2000, Vector Laboratories.

    Incubation:

    Article Title: Focal clusters of peri-synaptic matrix contribute to activity-dependent plasticity and memory in mice
    Article Snippet: .. The sections were then incubated in biotinylated anti-mouse IgM secondary antibody (Vector labs, BA-2020) 1 to 500 μL) for 2 h, followed by incubation in Streptavidin-HRP (Invitrogen, 434323) (1:5000) for another 2 h. Finally, tissue sections were washed with PB and incubated with 3,3’-Diaminobenzidine Tetrahydrochloride (Sigma, D5905–100TAB) 0.5 mg/mL in 0.1 M Sodium Cacodylate, 0.005% hydrogen peroxide for 20 min. ..



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